Enhancement of the In Vitro Root Regeneration Efficiency of Rehmannia glutinosa Libosch. Stem Explants by Different Carbon Sources
نویسندگان
چکیده
The success of plant tissue cultures is influenced by plant growth regulators, nutrient supply, and the carbon source in the medium. We investigated the most suitable carbon source and concentration for the in vitro rooting of Rehmannia glutinosa. Stem segments of R. glutinosa were cultured in vitro with 30 g/L of seven different carbon sources (sucrose, dextrose, mannose, glucose, sucrose, galactose, fructose, and maltose) for adventitious root regeneration. Subsequently, the best carbon source for regeneration was selected, and the optimal concentration (0%, 0.5%, 1.0%, 2.0%, 3.0%, 4.0%, or 5.0%) for promoting root regeneration was determined. Three percent sucrose was found to be the most suitable carbon source and concentration for adventitious root regeneration. [Thwe AA, Chae SC, Chung SO, Park SU. Enhancement of the In Vitro Root Regeneration Efficiency of Rehmannia glutinosa Libosch. Stem Explants by Different Carbon Sources. Life Sci J 2013;10(3):579-582] (ISSN:1097-8135). http://www.lifesciencesite.com. 85
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